Download Characterization of the Angiotensin receptor AT2 by various by Chirag Mandavia PDF

By Chirag Mandavia

booklet Date: February 22, 2013 | ISBN-10: 3659348147 | ISBN-13: 978-3659348143
In order to review AT2-mediated sign transduction mechanisms, major ways have been used: (1) the ligand-binding houses of mutant AT2 receptors have been characterised compared to wild-type after new release of some degree mutation utilizing site-directed mutagenesis, and (2) interacting protein companions have been analyzed utilizing yeast library screening, co-immunoprecipitation and Western Blot stories. We discovered that the facility of Angiotensin II (Ang II) receptor kind AT2 Phe308Ala residue mutant within the seventh TMD to bind peptidic ligands was once tremendously lowered, utilizing Xenopus oocytes expression method. this layer occupies a place within the AT2 analogous to the Tyr292 within the AT1 crucial for agonistic activation of that receptor. Screening of a 17-day mouse embryo cDNA library with the Angiotensin II receptor AT2 because the bait in yeast two-hybrid assay pointed out sodium-hydrogen exchanger NHE6 because the binding accomplice with ninety eight% series homology. extra reviews published that after human cell-line MCF-7 used to be transfected with the AT2, the AT2 co-immunoprecipitated with the NHE6 within the presence of Ang II, implying that Ang II-mediated activation is required for the interplay among AT2 and NHE6.
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